Persistence of cyprinid herpes virus 3 (CyHV-3) in infected cultured carp cells.
Dishon A, Davidovich M, Ilouze M, Kotler M
J Virol 2007 Feb 14.
Cyprinid herpes virus 3 (CyHV-3) previously designated carp interstitial nephritis and
gill necrosis virus (CNGV), and koi herpes virus (KHV), is the cause of a worldwide
mortal disease of koi and carp.
Morphologically the virus resembles herpes viruses, yet bears a genome of
277-295 kbp, which is divergent from most of the genomic sequences
available in the GenBank.
The disease afflicts fish at the transient seasons, when the water temperature is
18-28 degrees C, conditions which permit virus propagation in cultured cells.
Here we report that infectious virus is
preserved in cultured cells
maintained for 30 days at 30 degrees C. CyHV-3-infected vacuolated cells with
deformed morphology converted to normal, and plaques disappeared following
shifting up of the temperature,
and reappeared after transfer to the permissive temperature.
Viral propagation and viral gene transcription were turned off by shifting cells to
the non-permissive temperature.
Upon return of the cells to permissive temperature, transcription of viral genes
was reactivated in a sequence distinguished from that occurring in naïve cells
Our results show that CyHV-3 persists in cultured cells maintained at the
temperature and suggest that viruses could persist for long periods in the
enabling a new burst of infection upon shifting to permissive temperature.
record/17301126/abstract Persistence of cyprinid herpes virus 3 CyHV 3 in infected cultured carp cells.